Screening of Exotic Potato Germplasm against Potato Virus PVX, PVY and PLRV through Biological and Serological Test (ELISA)
نویسندگان
چکیده
منابع مشابه
Digoxigenin-labelled molecular probe for the simultaneous detection of three potato pathogens: potato spindle tuber viroid (PSTVd), potato virus Y (PVY), and potato leafroll virus (PLRV).
A molecular probe, p3POT, was constructed of PSTVd, PVY, PLRV cDNA fragments introduced into pUC18 vector. Sequencing of the inserts revealed that cloned fragments covered conservative parts of pathogenic genomes. Dot-blot hybridization of digoxigenin-labelled construct to crude extracts from plants infected with different potato viruses proved high sensitivity and specificity of the p3POT prob...
متن کاملBiological, serological and molecular typing of potato virus Y (PVY) isolates from Tunisia.
In Tunisia, potato virus Y (PVY) currently presents a significant threat to potato production, reducing tuber yield and quality. Three hundred and eighty-five potato samples (six different cultivars) collected in autumn 2007 from nine regions in Tunisia were tested for PVY infection by DAS-ELISA. The virus was detected in all regions surveyed, with an average incidence of 80.26%. Subsequently, ...
متن کاملdetection of pvy, pvx, pvs, pva, and plrv on different potato varieties in turkey using das-elisa
this research was performed in order to study and diagnose potato virus y,potyvirus (pvy), potato virus x,potexvirus (pvx), potato virus s,carlavirus (pvs), potato virus a,potyvirus(pva) and potato leafroll luteovirus (plrv) on tubers and leaves of different potato varieties, namely, solea, safran, floris, proventa, milva, universa, lady olympia, vangogh, and marabel grown in afyon region of tu...
متن کاملInfection Pattern of PVY and PLRV in Potato Tubers Obtained from Potato Plants Infected with Both the Viruses
Experiments were conducted to study the plant growth and tuber yield of the seed potato collected from PVY and PLRV infected plants. The seed potatoes were graded as under size (<28 mm), A grade ( 28-40 mm), B grade (41-55 mm) and over size (>55 mm) which were planted following RCBD in the field. Third generation seed potatoes of Diamant variety were used. The incidence of viruses was confirmed...
متن کاملPerformance of Seed Potato Produced from Sprout Cutting, Stem Cutting and Conventional Tuber against Pvy and Plrv*
The performance of seed tubers harvested from potato plants grown from sprout cutting, stem cutting and conventional seed tubers against PVY and PLRV were investigated. Sprout cutting, stem cutting and conventional seed tubers of variety Diamant were planted and second and third generation seed tubers were harvested. The least incidence of PVY and PLRV was recorded when seed tubers from sprout ...
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ژورنال
عنوان ژورنال: Sarhad Journal of Agriculture
سال: 2020
ISSN: 1016-4383
DOI: 10.17582/journal.sja/2020/36.3.986.991